The clinical applicability of a new automatic sugar analyser
نویسندگان
چکیده
Introduction The clinical laboratory is regularly faced with the task of establishing the causes of non-diabetic melituria. These causes may be: physiological for neonates ], caused by hereditary defects of carbohydrate metabolism [2], brought about by congenital or acquired deficiencies of disaccharidases.[3], or an abnormal sugar excretion may be found with enteritis [4]. The necessary analysis is often approached qualitatively by using thinlayer chromatography [5, 6, 7] ;this gives a quick result which is often sufficient. Ion exchange chromatography allows the quantitative determination of a range of carbohydrates in urine whereas enzymatic methods only measure single compounds. Column chromatographic methods previously described for use on clinical materials [8, 9, 10] suffer from disadvantages; long analytical run-times are required and the corrosive orcinol-conc, sulphuric acid reagent is used to detect any nonreducing sugars. Recently a method 11 usesthe non-corrosive Nanochrom II reagent, and is investigated here for its applicability to routine clinical analysis.
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ورودعنوان ژورنال:
- The Journal of Automatic Chemistry
دوره 3 شماره
صفحات -
تاریخ انتشار 1981